NICU room sample - SRA

SRX3242225: NICU room sample
1 ILLUMINA (Illumina MiSeq) run: 51.3M spots, 30.8G bases, 16.2Gb downloads

Design: Genomic DNA from room samples were subjected to 16S rRNA V3-4 MiSeq library preparation which included dual-barcoded multiplexing with a heterogeneity spacer for higher sequence quality [Fadrosh et al, Microbiome 2014]. Two microliters of 5X concentrated gDNA template was used in the reaction and run at 35 cycles. Amplicons were purified using the Just-a-Plate PCR normalization and purification kit (Charm Biotech, San Diego, CA, USA). Equal amounts of each sample were sent to the University of California Davis DNA Technologies Core Facility (http://dnatech.genomecenter.ucdavis.edu) and run on a MiSeq with v3 300PE chemistry.

Submitted by: Jill Banfield's Lab at Berkeley (BanfieldLab)

Study: Metagenomes from hospital (NICU) surfaces and sinks

PRJNA376580 • SRP103137 • All experiments • All runs

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This data was generated to recover genomes from hospital (NICU) surfaces and sinks.

Sample: NICU room sample

SAMN07641216 • SRS2565257 • All experiments • All runs

Organism: indoor metagenome

Library:

Name: SAMN07641216_to_SAMN07641407

Instrument: Illumina MiSeq

Strategy: AMPLICON